N; lowglucose culture remedy group; 400 M sodium hydrosulfite option + low-glucose culture resolution group; soon after cultivating for 30 min, 1 h, 2 h, four h and 24 h, respectively, replace with regular highglucose culture option, location in 37 and 5 CO2 incubator, and continue cultivating for 30 min, 1 h, 2 h, 4 h and 24 h, respectively[13,15,16]Preparation of drug-containing plasma Experimental groupingNUAIRETM US AUTOFLOW CO2 incubator (NUAIRE Corporation in Germany), AE31-type inverted fluorescence phase contrast microscope (Motic Enterprise), UNRISE microplate reader (TECAN Firm, in Switzerland). VDF-based microelectrode amplifiers (Shanghai Cathay Pacific Telecommunications Equipment Factory)Pharmacognosy Magazine | July-September 2013 | Vol 9 | IssueExperimental mice, traditional breeding, are offered water, but not food for half day prior to blood sampling. Experimental grouping: they may be randomly divided into six groups, 3 in each group: highRen, et al.: Protective effects of ferulic acid on principal cultured neonatal rat cardiomyocytesdose ferulic acid group: 7.eight mg/mL; medium dose ferulic acid group: 2.six mg/mL; lowdose ferulic acid group: 0.87 mg/mL; Chinese positive (Yangxinshi tablet) group: 0.0776 mg/mL; Western positive group (verapamil hydrochloride tablet): 0.0776 mg/mL; handle group: organic drinking water; filling into stomach for three days, 0.five mL/time, twice each day, take blood from eyeball, set Ep tube, prepare drugcontaining plasma and blank plasma, and retailer for use.882670-92-0 Order Preparation of drug-containing plasmaSurvival rate = OD test/OD handle, injury inhibition price = (OD testOD control)/OD control.Methyl 6-aminopicolinate Chemscene The detection of LDH activity inside the myocardial cell culture supernatantPut blood sample in Ep tube, that is added with heparin (concentration of 0.PMID:23927631 25 mg/mL) ahead of time, shake slowly, centrifuge at 1000 r/min for 15 min, extract the supernatant, that is the drugcontaining plasma, sub package, and place in 20 refrigerator for storage.Protective effects of ferulic acid on cardiomyocytes with hypoxia/reoxygenation injuryCatalytic lactic acid generate pyruvate, pyruvate and 2, 42 nitrobenzene hydrazine react and generate pyruvic acid nitrobenzene hydrazone, appear to become palm red inside the alkaline option. The enzyme activity is usually obtained by colorimetric method. Take the cell culture supernatant 50 L and test the tube absorbency worth in 440 nm immediately after reaction.The determination of Na+-K+- ATPase and Ca2+ATPase activity in the myocardial cellTake main cultured neonatal rat cardiomyocytes cultivated for 4 days and randomly divide into six groups, 10 pores every single group: blank control group: 400 uM sodium hydrosulfite for hypoxia/reoxygenation 2 h, and add into DMEM culture option; Chinese positive manage group: 0.0776 mg/mL Yangxinshi tablet; Western optimistic handle group: 0.0776 mg/mL verapamil hydrochloride tablet; unique ferulic acid concentration (800 M, 400 M and 200 M) groups; place into 37 and five CO2 incubator for cultivating for 24 h.Protective effects of drug-containing plasma on cardiomyocytes with hypoxia/reoxygenation injuryATPase can decompose ATP production ADP and inorganic phosphorus, by determination the volume of inorganic phosphorus we are able to know ATPase activity. Soon after the experiment, abandon the medium and digestive with mixed digested liquid 2 mL for 12 min, immediately after a number of cells fall off, abandon the digest fluid, add 3 mL medium, wind down the cell, centrifugal and wash a single time, add 1 mL PBS in to the cell, us.