M/z: [M + H]+ Calcd for C22H25N2O2 349.1911, Found 349.1905. (9H-Fluoren-9-yl)methyl 4-(two,5-dimethyl-1H-pyrrol-1-yl)phenethylcarbamate (17c)–Yield 243 mg (84 ); white crystals; mp = 215-218 ; Rf = 0.three (EtOAc/hexanes, 1:15-1:6); 1H NMR (500 MHz, CDCl3) 7.85 – 7.78 (d, J = 7.six Hz, 2H), 7.67 – 7.60 (d, J = 7.four Hz, 2H), 7.48 – 7.42 (t, J = 7.4 Hz, 2H), 7.40 – 7.32 (t, J = 7.4 Hz, 2H), 7.31 – 7.24 (d, J = six.7 Hz, 2H), 7.18 (s, 1H), 5.95 (s, 2H), 4.90 (s, 1H), four.49 (d, J = six.7 Hz, 2H), four.27 (t, J =J Org Chem. Author manuscript; accessible in PMC 2014 November 01.Walia et al.Page6.six Hz, 1H), three.54 (q, J = six.6 Hz, 2H), two.91 (t, J = 6.9 Hz, 2H), 2.11 (s, 6H); 13C NMR (126 MHz, CDCl3) 156.three, 143.9, 141.four, 138.3, 137.four, 129.5, 128.9, 128.three, 127.eight, 127.1, 125.0, 120.0, 105.6, 66.five, 47.three, 42.2, 35.9, 13.1; HRMS (ESI-TOF) m/z: [M + H]+ Calcd for C29H29N2O2 437.2224, Identified 437.2226. Compounds 18a-c had been synthesized by General Process B: tert-Butyl 4-aminophenethylcarbamate (18a)–Yield 56.4-Chloropyridazin-3-ol Price four mg (67 ); clear oil; Rf = 0.Price of 2619509-30-5 three (EtOAc/hexanes, 1:15-1:6); 1H NMR (500 MHz, CDCl3) 6.98 (d, J = 7.9 Hz, 2H), 6.64 (d, J = 7.9 Hz, 2H), four.68 (bs, 1H), three.56 (bs, 2H), 3.32 (q, J = 6.7 Hz, 2H), 2.PMID:24576999 68 (t, J = 7.two Hz, 2H), 1.45 (s, 9H); 13C NMR (126 MHz, CDCl3) 156.0, 144.9, 129.6, 128.eight, 115.4, 79.1, 42.1, 35.two, 28.5; LRMS (ESI): m/z = 259.09 [M + Na]+. The information were in accordance with those previously reported.28 Benzyl 4-aminophenethylcarbamate (18b)–Yield 56.six mg (77 ); clear oil; Rf = 0.3 (EtOAc/hexanes, 1:15-1:six); 1H NMR (500 MHz, CDCl3) 7.50 – 7.33 (m, 5H), 6.99 (d, J = eight.0 Hz, 2H), six.62 (d, J = 8.0 Hz, 2H), 5.13 (s, 2H), 3.63 (bs, 2H), three.42 (m, 2H), 2.72 (m, 2H); 13C NMR (126 MHz, CDCl3) 156.5, 145.1, 136.eight, 129.7, 128.6, 128.five, 128.2, 127.9, 115.4, 66.six, 42.six, 35.2; LRMS (ESI): m/z = 293.13 [M + Na]+. The information had been in accordance with these previously reported.29 (9H-Fluoren-9-yl)methyl 4-aminophenethylcarbamate (18c)–Yield 78.two mg (78 ); colorless oil; Rf = 0.3 (EtOAc/hexanes, 1:10-1:4); 1H NMR (500 MHz, CDCl3) 7.77 (d, J = 7.five Hz, 2H), 7.58 (d, J = 7.five Hz, 2H), 7.40 (t, J = 7.five Hz, 2H), 7.32 (m, 2H), 6.96 (d, J = 7.7 Hz, 2H), 6.64 (d, J = 7.eight Hz, 2H), 4.39 (d, J = 6.9 Hz, 2H), four.23 (d, J = 7.two Hz, 1H), three.61 (bs, 2H), 3.40 (d, J = 6.five Hz, 2H), 2.72 (t, J = 7.0 Hz, 2H); 13C NMR (126 MHz, CDCl3) 156.three, 144.0, 141.3, 129.7, 128.six, 127.7, 127.0, 125.1, 120.0, 115.4, 66.5, 53.five, 47.three, 42.5, 35.2; LRMS (ESI): m/z = 381.20 [M + Na]+. The information had been in accordance with these previously reported.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptSupplementary MaterialRefer to Web version on PubMed Central for supplementary material.AcknowledgmentsThe authors are grateful for the National Institutes of Health (Grant GM049725) for generous monetary support of this study.
Identification of Crucial Genes for Growth in Olive Brine by Transposon Mutagenesis of Lactobacillus pentosus CG. Perpetuini,a,b H. Scornec,b R. Tofalo,a P. Serror,d,e M. Schirone,a G. Suzzi,a A. Corsetti,a J. F. Cavin,b H. Licandro-Serautb,cDipartimento Scienze degli Alimenti, Universit?di Teramo, Teramo, Italya; UMR A 02102 PAM, AgroSup Dijon, and Universit?de Bourgogne, Dijon, Franceb; Unit?de Pathog ie Microbienne Mol ulaire, Institut Pasteur, Paris, Francec; INRA, UMR1319 Micalis, Jouy-en-Josas, Franced; AgroParisTech, UMR Micalis, Jouy-en-Josas, FranceeOlive brine represents a stressful environment on account of the high NaCl concentration, presence of phenolic compounds identified.
